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polyclonal rabbit anti gpr161 proteintech 13398 1 ap  (Proteintech)


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    Structured Review

    Proteintech polyclonal rabbit anti gpr161 proteintech 13398 1 ap
    Polyclonal Rabbit Anti Gpr161 Proteintech 13398 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+gpr161+proteintech+13398+1+ap/GPR161+Antibody/pmc10320715__biolopen___12___059976___s1-17-51-54
    Average 93 stars, based on 62 article reviews
    polyclonal rabbit anti gpr161 proteintech 13398 1 ap - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Molecular basis underlying the ciliary defects caused by IFT52 variations found in skeletal ciliopathies
    Article Snippet: Antibodies used in this study Antibody Manufacturer or provider Clone/catalog number or reference Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:1,000 (IF, IB) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Monoclonal mouse anti-Ac-α-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU-88 1:500 (IF) Polyclonal rabbit anti-GFP MBL 598 1:1000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Polyclonal rabbit anti-IFT70 Proteintech 25352-1-AP 1:1000 (IB) Monoclonal mouse anti-β-Tubulin Proteintech 66240-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21127, A21131, A21147, A21240, A21242 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB)

    Article Title: Dynein-2–driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex
    Article Snippet: Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT25 Proteintech 15732-1-AP 1:1,000 (IB) Polyclonal rabbit anti-IFT52 Proteintech 17534-1-AP 1:1,000 (IB) Polyclonal rabbit anti-IFT81 Proteintech 11744-1-AP 1:1,000 (IB) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF), 1:1,000 (IB) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:500 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Monoclonal mouse anti-Smoothened Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-GAPDH Ambion 6C5 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21127, A21131, A21147, A21241, A21242, A21245 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB)

    Article Title: CCRK/CDK20 regulates ciliary retrograde protein trafficking via interacting with BROMI/TBC1D32
    Article Snippet: Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-α-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:5,000 (IF), 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB)

    Immunofluorescence:

    Article Title: BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to intraflagellar transport turnaround involving ICK/CILK1
    Article Snippet: .. Antibodies used in this study Antibody Manufacturer or provider Clone/catalog number or reference Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-αtubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Polyclonal rabbit anti-tagRFP Evrogen AB233 1:1,000 (IB) Monoclonal mouse anti-β-tubulin Proteintech 66240-1-Ig 1:20,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21131, A21147, A21137, A21242, A21240 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting Table S3. ..

    Article Title: ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane
    Article Snippet: Plasmids used in this study Vector Insert Reference pCAG2-EGFP-C INPP5E (Nozaki et al., 2017) pCAG2-EGFP-C INPP5E (1–626) This study pCAG2-EGFP-C INPP5E(∆CTS) (Qiu et al., 2021) pCAG2-mCherry-C INPP5E (Nozaki et al., 2017) pCAG2-mCherry-C INPP5E (1–626) This study pCAG2-mCherry-C INPP5E(∆CTS) (Qiu et al., 2021) pCAG2-tBFP2-C INPP5E This study pCAG-ds-EGFP-N ARL13B (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B(T35N) (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B (R79Q) (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B (Y86C) This study pCAG-ds-EGFP-N ARL13B (R200C) (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B (AAEA) (Nozaki et al., 2017) pCAG2-mCherry-N ARL13B (Nozaki et al., 2017) pCAG-ds-tBFP2-N ARL13B This study pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B(T35N) (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (R79Q) (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (Y86C) This study pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (R200C) (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (AAEA) (Nozaki et al., 2017) pEGFP-N ARL3 This study pcDNA3-mCherry-N ARL3 This study pTagBFP2-N ARL3 This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL3 This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL3 (QL) This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL3 (TN) This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL13B This study pEGFP-C1 PDE6D This study pmCherry-C1 PDE6D This study pTagBFP2-C PDE6D This study pDonor-tBFP-NLS-Neo(universal) - (Katoh et al., 2017) pHiFiCas9-2xsgRNA - This study pGEX-6P1 Anti-GFP-nanobody (Katoh et al., 2015) B io lo gy O pe n • S up pl em en ta ry in fo rm at io n Table S2. .. Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-TULP3 Proteintech 136371-AP 1:200 (IF) Polyclonal rabbit anti-INPP5E Proteintech 17797-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:500 (IF) Polyclonal rabbit anti-ARL6 Proteintech 12676-1-AP 1:500 (IF) Polyclonal rabbit anti-BBS9 Atlas Antibodies HPA021289 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-Ac-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:5,000 (IB) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21429, A21137, A21127, A21240, A21147, A21131, A21242 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting. ..

    Article Title: CCRK/CDK20 regulates ciliary retrograde protein trafficking via interacting with BROMI/TBC1D32
    Article Snippet: Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-α-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:5,000 (IF), 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) .. reference Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-α-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:5,000 (IF), 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21131, A21147, A21137, A21242, A21240 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting ..

    Article Title: Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport
    Article Snippet: .. Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:500 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Monoclonal mouse anti-Smoothened Santa Cruz sc-166685 1:100 (IF) Polyclonal goat anti-CEP164 Santa Cruz sc-240226 1:500 (IF) Monoclonal mouse anti-acetylated-a-tubulin Sigma-Aldrich 6-11-B 1:2000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21127, A21241, A21131, A21242, A21245 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting Jo ur na l o f C el l S ci en ce • S up pl em en ta ry in fo rm at io n ..

    Western Blot:

    Article Title: BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to intraflagellar transport turnaround involving ICK/CILK1
    Article Snippet: .. Antibodies used in this study Antibody Manufacturer or provider Clone/catalog number or reference Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-αtubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Polyclonal rabbit anti-tagRFP Evrogen AB233 1:1,000 (IB) Monoclonal mouse anti-β-tubulin Proteintech 66240-1-Ig 1:20,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21131, A21147, A21137, A21242, A21240 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting Table S3. ..

    Article Title: ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane
    Article Snippet: Plasmids used in this study Vector Insert Reference pCAG2-EGFP-C INPP5E (Nozaki et al., 2017) pCAG2-EGFP-C INPP5E (1–626) This study pCAG2-EGFP-C INPP5E(∆CTS) (Qiu et al., 2021) pCAG2-mCherry-C INPP5E (Nozaki et al., 2017) pCAG2-mCherry-C INPP5E (1–626) This study pCAG2-mCherry-C INPP5E(∆CTS) (Qiu et al., 2021) pCAG2-tBFP2-C INPP5E This study pCAG-ds-EGFP-N ARL13B (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B(T35N) (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B (R79Q) (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B (Y86C) This study pCAG-ds-EGFP-N ARL13B (R200C) (Nozaki et al., 2017) pCAG-ds-EGFP-N ARL13B (AAEA) (Nozaki et al., 2017) pCAG2-mCherry-N ARL13B (Nozaki et al., 2017) pCAG-ds-tBFP2-N ARL13B This study pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B(T35N) (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (R79Q) (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (Y86C) This study pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (R200C) (Nozaki et al., 2017) pRRLsiPPT-tRFP-T-N-IRES-Zeo ARL13B (AAEA) (Nozaki et al., 2017) pEGFP-N ARL3 This study pcDNA3-mCherry-N ARL3 This study pTagBFP2-N ARL3 This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL3 This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL3 (QL) This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL3 (TN) This study pRRLsinPPT-EGFP-N--IRES-Zeo ARL13B This study pEGFP-C1 PDE6D This study pmCherry-C1 PDE6D This study pTagBFP2-C PDE6D This study pDonor-tBFP-NLS-Neo(universal) - (Katoh et al., 2017) pHiFiCas9-2xsgRNA - This study pGEX-6P1 Anti-GFP-nanobody (Katoh et al., 2015) B io lo gy O pe n • S up pl em en ta ry in fo rm at io n Table S2. .. Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-TULP3 Proteintech 136371-AP 1:200 (IF) Polyclonal rabbit anti-INPP5E Proteintech 17797-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:500 (IF) Polyclonal rabbit anti-ARL6 Proteintech 12676-1-AP 1:500 (IF) Polyclonal rabbit anti-BBS9 Atlas Antibodies HPA021289 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-Ac-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:5,000 (IB) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21429, A21137, A21127, A21240, A21147, A21131, A21242 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting. ..

    Article Title: CCRK/CDK20 regulates ciliary retrograde protein trafficking via interacting with BROMI/TBC1D32
    Article Snippet: Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-α-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:5,000 (IF), 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) .. reference Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:5,000 (IF) Monoclonal mouse anti-Ac-α-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:1,000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:5,000 (IF), 1:10,000 (IB) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21131, A21147, A21137, A21242, A21240 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting ..

    Article Title: Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport
    Article Snippet: .. Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:200 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:500 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Monoclonal mouse anti-Smoothened Santa Cruz sc-166685 1:100 (IF) Polyclonal goat anti-CEP164 Santa Cruz sc-240226 1:500 (IF) Monoclonal mouse anti-acetylated-a-tubulin Sigma-Aldrich 6-11-B 1:2000 (IF) Monoclonal mouse anti-RFP MBL 3G5 1:1,000 (IF) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A21127, A21241, A21131, A21242, A21245 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting Jo ur na l o f C el l S ci en ce • S up pl em en ta ry in fo rm at io n ..

    Modification:

    Article Title: Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry
    Article Snippet: B io lo gy O pe n • S up pl em en ta ry in fo rm at io n 3 Vector Insert Reference pCAG2-EGFP-C INPP5E (Nozaki et al., 2017) pCAG2-mCherry-N ARL13B (Nozaki et al., 2017) pCAG2-EGFP-C INPP5E(D477N) This study pCAG2-EGFP-C INPP5E(∆CTS) This study pRRLsinPPT-EGFP-C-IRES-Zeo INPP5E This study pRRLsinPPT-EGFP-C-IRES-Zeo INPP5E(D477N) This study pRRLsinPPT-EGFP-C-IRES-Zeo INPP5E(∆CTS) This study pRRLsinPPT-EGFP-C-IRES-Zeo TULP3 (Nozaki et al., 2017) pRRLsinPPT-EGFP-C-IRES-Blast MKS1 (Okazaki et al., 2020) pRRLsinPPT-mCh-FRB-N-IRES-Zeo SSTR3 This study pRRLsinPPT-FKBP-EGFP-C-IRES-Zeo INPP5E This study pRRLsinPPT-FKBP-EGFP-C-IRES-Zeo INPP5E(∆CTS) This study pGEX-6P1 Anti-GFP-nanobody (Katoh et al., 2015) B io lo gy O pe n • S up pl em en ta ry in fo rm at io n 4 Table S2. .. Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Polyclonal rabbit anti-INPP5E Proteintech 17797-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:500 (IF) Polyclonal rabbit anti-TCTN1 Proteintech 15004-1-AP 1:100 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-Ac-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:500 (IF) Monoclonal mouse anti-polyglutamylation modification AdipoGen GT335 1:500 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Polyclonal rabbit anti-GFP Invitrogen A11122 1:10,000 (IF) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A27039, A21244, A11004, A21127, A21240, A21241, A21131, A21242 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting B io lo gy O pe n • S up pl em en ta ry in fo rm at io n 5 Name Sequence INPP5E -genome-FW (primer 1) 5'- CGTCCAAGGCGGAGAATCTG-3' INPP5E -genome-RV (primer 2) 5'- TTGTAGTCTGCAAGATCCGAGTC-3' pTagBFP-N-RV2 (primer 3) 5'- CGTAGAGGAAGCTAGTAGCCAGG -3' SLiCE-INPP5E-D477N-S 5'- GGTTTGGAAACTTCAACTTCCGCCTG-3' SLiCE-INPP5E-D477N-AS 5'- GTTGAAGTTTCCAAACCAGAACACCTC-3' SLiCE-INPP5E-delCTS-S 5'- AGCTGGGCAAACTAGGAATTAAAAGACGGATTTC-3' SLiCE-INPP5E-delCTS-AS 5'-TAATTCCTAGTTTGCCAGCTGCCAAC-3' SLiCE-IRES-FKBP-S 5'-GATGATAAGCTTGCCACAAGCCACCATGGGAGTGCAG-3' SLiCE-IRES-INPP5E-AS 5'-TGTAATCCAGAGGTTGATTTCAAGAAACGGAGCAGATGG-3' INPP5E-gRNA#1-S 5'- CACCCCTGGACCCCGATGACATAC-3' INPP5E-gRNA#1-AS 5'- AAACGTATGTCATCGGGGTCCAGG-3' INPP5E-gRNA#2-S 5'- CACCGGAAATCCCCAAGTCCCGCG-3' INPP5E-gRNA#2-AS 5'- AAACCGCGGGACTTGGGGATTTCC-3' ..

    Sequencing:

    Article Title: Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry
    Article Snippet: B io lo gy O pe n • S up pl em en ta ry in fo rm at io n 3 Vector Insert Reference pCAG2-EGFP-C INPP5E (Nozaki et al., 2017) pCAG2-mCherry-N ARL13B (Nozaki et al., 2017) pCAG2-EGFP-C INPP5E(D477N) This study pCAG2-EGFP-C INPP5E(∆CTS) This study pRRLsinPPT-EGFP-C-IRES-Zeo INPP5E This study pRRLsinPPT-EGFP-C-IRES-Zeo INPP5E(D477N) This study pRRLsinPPT-EGFP-C-IRES-Zeo INPP5E(∆CTS) This study pRRLsinPPT-EGFP-C-IRES-Zeo TULP3 (Nozaki et al., 2017) pRRLsinPPT-EGFP-C-IRES-Blast MKS1 (Okazaki et al., 2020) pRRLsinPPT-mCh-FRB-N-IRES-Zeo SSTR3 This study pRRLsinPPT-FKBP-EGFP-C-IRES-Zeo INPP5E This study pRRLsinPPT-FKBP-EGFP-C-IRES-Zeo INPP5E(∆CTS) This study pGEX-6P1 Anti-GFP-nanobody (Katoh et al., 2015) B io lo gy O pe n • S up pl em en ta ry in fo rm at io n 4 Table S2. .. Antibodies used in this study Antibody Manufacturer Clone/catalog number or reference number Dilution (purpose) Polyclonal rabbit anti-IFT88 Proteintech 13967-1-AP 1:500 (IF) Polyclonal rabbit anti-IFT140 Proteintech 17460-1-AP 1:500 (IF) Polyclonal rabbit anti-ARL13B Proteintech 17711-1-AP 1:1,000 (IF) Polyclonal rabbit anti-INPP5E Proteintech 17797-1-AP 1:500 (IF) Polyclonal rabbit anti-GPR161 Proteintech 13398-1-AP 1:500 (IF) Polyclonal rabbit anti-TCTN1 Proteintech 15004-1-AP 1:100 (IF) Monoclonal mouse anti-ARL13B Abcam N295B/66 1:500 (IF) Monoclonal mouse anti-SMO Santa Cruz sc-166685 1:100 (IF) Monoclonal mouse anti-Ac-tubulin Sigma-Aldrich 6-11B-1 1:1,000 (IF) Monoclonal mouse anti-γ-tubulin Sigma-Aldrich GTU88 1:500 (IF) Monoclonal mouse anti-polyglutamylation modification AdipoGen GT335 1:500 (IF) Monoclonal mouse anti-FOP Abnova 2B1 1:10,000 (IF) Polyclonal rabbit anti-GFP Invitrogen A11122 1:10,000 (IF) Polyclonal rabbit anti-mCherry Proteintech 26765-1-AP 1:10,000 (IB) Monoclonal mouse anti-GFP Proteintech 66002-1-Ig 1:10,000 (IB) AlexaFluor-conjugated secondary Molecular Probes A11034, A27039, A21244, A11004, A21127, A21240, A21241, A21131, A21242 1:1,000 (IF) Peroxidase-conjugated secondary Jackson ImmunoResearch 115-035-166, 111-035-144 1:3,000 (IB) IF, immunofluorescence; IB, immunoblotting B io lo gy O pe n • S up pl em en ta ry in fo rm at io n 5 Name Sequence INPP5E -genome-FW (primer 1) 5'- CGTCCAAGGCGGAGAATCTG-3' INPP5E -genome-RV (primer 2) 5'- TTGTAGTCTGCAAGATCCGAGTC-3' pTagBFP-N-RV2 (primer 3) 5'- CGTAGAGGAAGCTAGTAGCCAGG -3' SLiCE-INPP5E-D477N-S 5'- GGTTTGGAAACTTCAACTTCCGCCTG-3' SLiCE-INPP5E-D477N-AS 5'- GTTGAAGTTTCCAAACCAGAACACCTC-3' SLiCE-INPP5E-delCTS-S 5'- AGCTGGGCAAACTAGGAATTAAAAGACGGATTTC-3' SLiCE-INPP5E-delCTS-AS 5'-TAATTCCTAGTTTGCCAGCTGCCAAC-3' SLiCE-IRES-FKBP-S 5'-GATGATAAGCTTGCCACAAGCCACCATGGGAGTGCAG-3' SLiCE-IRES-INPP5E-AS 5'-TGTAATCCAGAGGTTGATTTCAAGAAACGGAGCAGATGG-3' INPP5E-gRNA#1-S 5'- CACCCCTGGACCCCGATGACATAC-3' INPP5E-gRNA#1-AS 5'- AAACGTATGTCATCGGGGTCCAGG-3' INPP5E-gRNA#2-S 5'- CACCGGAAATCCCCAAGTCCCGCG-3' INPP5E-gRNA#2-AS 5'- AAACCGCGGGACTTGGGGATTTCC-3' ..



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